Created and under current development by Kjeld Olesen of Acaclone Software. pDRAW 32 accepts most file format including GCG, EMBL, fasta etc. It uses enzyme.dat, hence can be easily replaced with a updated copy (from GCG). Sequences can also be easily cut & paste into a new or existing file. In addition it will allow edits to sequence files. However the graphics are not touch sensitive and although it can produce coloured plots (and prints), this only applies to the restriction sites and annotation boxes can only be produced in black and white. The help pages are very poor, but it contains very nice science help with PCR and cloning protocols and a lovely coloured periodic table. It is worth downloading, for its oligo calculator, ORF and mapping facility. Since my review, newer revisions of pDRAW32 have improves circular plotting of plasmids, specifically the positioning of restriction sites when many sites are selected. Since revision 1.0.35 pDRAW32 can track changes to the DNA sequence during sequence editing and move to positions of any annotations accordingly. The current revision has improved plotting quality and can analyse for and plot (or list) oligonucleotide priming sites.
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